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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
KillerTRAIL Storage and Dilution BufferFor Prod.No. 201-073 / 201-115 / 201-130
- 库存：
1
- 供应商：
上海甄准
- 规格：
500 uL
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文献和实验How to Make Simple Solutions and Dilutions
2 * DF3 etc. Example: In the microbiology lab in Bio 201 the students perform a three step 1:100 serial dilution of a bacterial culture (see figure below). The initial step combines 1 unit volume culture (10 ul) with 99 unit volumes
to the volume of trypsin. For cells that grow in clumps centrifuge and resuspend in a small volume and gently pipette to break up clumps. Under sterile conditions remove 100-2005l of cell suspension. Add an equal volume of Trypan Blue (Prod. No. T8154) (dilution factor
Subculture of Suspension Cell Lines
volume of Trypan Blue (Prod. No. T8154 ) (dilution factor =2) and mix by gentle pipetting. Clean the haemocytometer. Moisten the coverslip with water or exhaled breath. Slide the cover-slip over the chamber back and forth using slight pressure until Newton
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