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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Pentachloroaniline 100µg/mL
- 库存:
1
- 供应商:
甄准生物
- CAS号:
527-20-8
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文献和实验A general protocol for staining cell for cytometry analysis
6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture tube. 3. Add 5-15 µg of purified recombinant Annexin V. The amount of purified recombinant Annexin V required to saturate binding sites may vary
lysis buffer (high salt) ) 2 x 1 ml CHIP wash buffer 2 x 1 ml TE elute immunoprecipitations: add 75 µl elution buffer incubate for 10 min at 65 °C spin, take supernatant, elute pellet again with 75 µl
A general protocol for staining cell for cytometry analysis
-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1× Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 13024D) into 100 µl of 1× Binding Buffer and add to the cell pellet. Gently mix the cells. Add
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