mCD4 APC MAb (Cl GK1.5) (100 T

流式试剂

3 mAb,PE-Cy5 50T/100T   1200/1800  L0005 Mouse anti-Human CD3 mAb,APC 50T/100T   1000/1500  L0006 Mouse anti-Human CD3 mAb,Biotin  50T/100T

Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content

and one mAb directly conjugated to allophycocyanin (APC) for combining dual-color immunofluorescence with DNA-RNA staining. See Troubleshooting and Discussion for further information on strategies for fluorochrome selection and staining. Nucleic

Isolation of Non-muscle Actin

, 1 mM DTT, 0.5 mM MgCl2, 0.2 mM PMSF (add just before use). Buffer D: 10 mM Tris-Cl, pH 8.0, 0.2 mM CaCl2 , 0.5 mM ATP, 0.1 mM DTT, 100 mM KCl, 0.1 mM PMSF. Buffer G: 1 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 0.2 mM CaCl2 , 0.1 mM DTT, 0.005% NaN3 2. Make