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50 ligations
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文献和实验"Glassing Out" DNA Using the Geneclean Kit
. If there is more than 5 ug of DNA present, then 10 ul of Glassmilk suspension should be used. 7. Place tube in the Labquake rotator and let rotate for 5 minutes at room temperature. The DNA binds to the silica matrix during this step. 8. Pellet the silica matrix
Buffer or water to the tube. Incubate the tube at Resuspend MagSi particles by vortexing. Incubate at 65°C for 10 minutes if maxium DNA yield is desired. 19. Place the tube onto a magnetic separation device to magnetize the Mag-Bind? particles
concentration: Use 20 ng of insert DNA, 3 µL 5x Invitrogen ligation buffer, ligase and q.s. to 15 µL with water. Use 1 µL of enzyme in the first tube and then perform 10-fold serial dilutions of enzyme in 5 tubes. Incubate at room temperature for 15 minutes
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