- ￥3280
- Elabscience
- E-KAB-0625
- 武汉
- 2026年03月27日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 应用：
ELISA
- 适应物种：
Rat
- 规格：
50μL&100μg
| Synonyms |
CXCL3;CINC-2b;GRO3;GROg;MIP-2b;MIP2B;SCYB3
|
| Swissprot |
Q10746
|
| Reactivity |
Rat
|
| Buffer |
PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5; PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5
|
| Storage |
-20℃
|
| Expiration date |
12个月
|
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文献和实验,000 x g (9000 rpm)for 10 min at 4℃ in a SS-34 rotor. 5.In the cold room,collect the supernatant,transfer it to 31.5 mlμltracentrifuge tubes,pair them by weight,and clarify the cytosol by centrifugation at 100,000 x g (29,000 rpm)for 60 min at 4℃
Preparation of Rat Liver Cell Cytosol
In the cold room, collect the supernatant, transfer it to 31.5 ml ultracentrifuge tubes, pair them by weight, and clarify the cytosol by centrifugation at 100,000 x g (29,000 rpm) for 60 min at 4oC in a 50.2Ti rotor. Collect the clarified cytosolic
Identification of a Mutant Kinase/ATP Analog Pair
(if [y-32 P]ATP analog is used) or immunoblotted with a phosphospecific antibody (if available) to determine the extent of substrate phosphorylation. The kinase/ATP analog pair that provides the best phosphorylation of the substrate is then used
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