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- Elabscience
- E-KAB-0552
- 武汉
- 2026年03月27日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 应用：
ELISA
- 适应物种：
Mouse
- 规格：
50μL&100μg
| Synonyms |
C7;IFI10;INP10;SCYB10;crg-2;gIP-10;mob-1
|
| Swissprot |
P17515
|
| Reactivity |
Mouse
|
| Buffer |
PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5; PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5
|
| Storage |
-20℃
|
| Expiration date |
12个月
|
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Identification of a Mutant Kinase/ATP Analog Pair
(if [y-32 P]ATP analog is used) or immunoblotted with a phosphospecific antibody (if available) to determine the extent of substrate phosphorylation. The kinase/ATP analog pair that provides the best phosphorylation of the substrate is then used
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
, pour out 2 x petri dishes of PBS in the hood. Pregnant mouse is killed by cervical dislocation. (This is not done in the hood but on clean benchcote). Lay mouse out on its back and swab belly with 70% ethanol. With a pair of scissors (not sterile) nip
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