MELON GEL MONOCLONAL IGG

IgG Purification

Several immunological procedures can be successfully carried out using nonpurified antibodies, such as unfractionated antisera or ascitic fluid/culture supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result

Western Blotting with Monoclonal Ant

must be below 0.1% to avoid interference with the colorimetric reading).  For Protein Gel Electrophoresis of Cell Culture (without determining protein concentration) 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS

Purification of mAb (IgG)

1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH