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- 2026年03月27日
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文献和实验Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA?either natural or formed from oligonucleotides?containing a high?affinity site for a DNA?binding protein provide a powerful tool for purification. The biotin/streptavidin purification system is based on the tight and essentially irreversible
?streptavidin conjugates are synthesized by covalent coupling of thiol?modified DNA oligonucleotides and streptavidin (STV). The resulting conjugates have binding capacities for four equivalents of biotin and a complementary nucleic acid sequence. The conjugates
to create fusiontagged bait proteins and bait-specific antibodies are not available, bait proteins can be biotinylated and detected with labeled avidin or streptavidin. Pierce offers a full line of biotinylation reagents and enzyme-labeled avidin
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