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文献和实验Conventional polymerase chain reaction (PCR) enables reliable amplification of 3–4 kb of DNA (1 ) while attempts at optimisation has enabled 15.6 kb of λ DNA to be amplified (2 ). The maximum amplifiable length of PCR is limited by the low
Long-Range Polymerase Chain Reaction
Conventional polymerase chain reaction (PCR) enables reliable amplification of 3–4 kb of DNA (1 ) while attempts at optimization has enabled 15.6 kb of λ DNA to be amplified (2 ). The maximum amplifiable length of PCR is limited by the low
Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells
transgene under powerful baculovirus promoter leads to re-protein production in high quantities. Although the replication of AcMNPV is highly insect specific in nature, it can penetrate and transduce a wide range of cells of other origin. Efficient
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