5-THIO-MODIFIER C6 S-S

GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins

Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia, PA 19111 Jason R. Orlinck Brigham & Women"s Hospital, Boston, MA 02115 Excerpted from Protein

Oligodeoxynucleotide Containing S‐Functionalized 2′‐Deoxy‐6‐Thioguanosine: Facile Tools for Base‐Selective and Site‐Specific Internal Modification of

oligonucleotides with enzyme, photochemistry, or catalytic DNA. The functionality?transfer ODN (FT?ODN), which contains 2??deoxy?6?thioguanosine (6?thio?dG) functionalized with the 2?methyliden?1,3?diketone group, is hybridized with the target RNA to trigger

Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors

preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows