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Store at 4˚C in dark for 6 months
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase gene, PLAP (Kam et al 1985), is perhaps the most useful as it is very heat stable and is resistant to some chemical inhibitors that are active on other endogenous alkaline phosphatases. PLAP has been used as a histochemical reporter fairly recently
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
One of the most common methods in immunohistochemistry involves the use of an antibody to the antigen of interest detected indirectly with an enzymelabeled antispecies secondary antibody. The enzyme catalyzes the formation of a colored
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