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-20°C
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999
- 供应商:
广州威佳科技有限公司
- 规格:
1 x 96-Well Microplate Kit/2 x 96-Well Microplate Kit/5 x 96-Well Microplate Kit
| 规格: | 1 x 96-Well Microplate Kit | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 2 x 96-Well Microplate Kit | 产品价格: | ¥6480.0 |
| 规格: | 5 x 96-Well Microplate Kit | 产品价格: | ¥14400.0 |
RayBiotech is a leading life sciences company providing proteomic discovery tools. RayBiotech has nearly 150 employees whose objectives are to provide excellent products and service worldwide to RayBiotech customers which include scientists in industry, academic and research institutes in 41 countries. RayBiotech is committed to accelerating customer success through innovation and leadership in the Life Sciences.
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内,每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激,我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤,分别固定,打孔,猝灭,封闭,然后一抗孵育,二抗孵育,最后显色,用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正,最后得出以上数据。Quantitative
SH2 Domain-Based Tyrosine Phosphorylation Array
SH2 domain proteins on a membrane. The array assay is straightforward with no expensive equipment or radioactivity required: the cell lysate is incubated with the array membrane, and the signal is measured using a chemiluminescence-based detection system.
Activity-Based Protein Profiling of Protein Tyrosine Phosphatases
form of these enzymes. In this work, we will highlight the methods used to characterize activity-based probes as to their utility in biological contexts. Specifically, we will address activity-based protein profiling of the protein tyrosine phosphatases
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