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- abx069524
- 2026年03月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
参照说明书
- 英文名：
Mouse Tubulin Epsilon (TUBe) Protein
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
10 μg///50 μg///100 μg
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Assemble all the equipment and place 200 µl of sample in one tube. Set up a second gel containing 200 µl of protein molecular weight standards. Electrophoresis is carried out at 3 mA per gel until the bromophenol blue dye reaches
, the more concentrated the peak of elution of tubulin protein. DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport back to the laboratory.
per hour collecting 0.5 - 1 mL fractions. The first eluate is purified tubulin protein. Do not mix with later peak fractions, which contain microtubule-associated proteins (MAPs). Pool the purified tubulin +/- MAPs, divide into 0.5 mL aliquots, freeze
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