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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
Miniprep/Kit-free high-throughput protocol
centrifuge at maximum speed at 4 ˚C. Remove pellet from each tube with a toothpick. The cellular debris should stick well to the toothpick. Try to insert and remove the toothpick from the center of the tube so you don''t get any cellular debris
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