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- Bio-Rad伯乐生命科学
- 美国
- 5000117
- 2026年03月21日
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文献和实验Extraction of DNA using DNAzol® Reagent
before extraction with DNAzol® Reagent. 2. Centrifugation (optional): Sediment the homogenate for 10 min at 10,000 × g at 4°C or room temperature. Following centrifugation, transfer the resulting viscous supernatant to a fresh tube. This step removes
)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
How to Make Simple Solutions and Dilutions
as percent concentrations as weight per volume for dry reagent OR volume per volume for solutions. When working with a dry reagent it is mixed as dry mass (g) per volume and can be simply calculated as the concentration (expressed as a proportion or ratio) x
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