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文献和实验实验原理 Add a mixture of biotinylated monoclonal antibodies against non-DC cells to your starting sample. Add Depletion MyOne™ SA Dynabeads® and allow them to bind to the non-DCs during a short incubation. Separate
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
7) Repeat Step 6 with another 700 ul of SPW Wash buffer diluted with ethanol. 8) Centrifuge at maximum speed ($13,000 x g) for 2 minute to dry the column. Discard the 2 mL collection tubes. 9) Place the MicroElute™ HiBind™ DNA
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