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文献和实验M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin.1M Hepes: 119.1 g Hepes + 500 ml dH2 O.Media A: 1X HBSS (10X:50ml) + 10 mM Hepes (1M: 5 ml) + 5 mM EDTA (0.5M: 5ml) + 2.5 % FBS (12.5 ml) in 500 ml.Media B: RPMI1640 470 ml + Gent
Purification of human mononuclear cells and neutrophils
(1M: 5 ml) + 5 mM EDTA (0.5M: 5ml) + 2.5 % FBS (12.5 ml) in 500 ml. Media B: RPMI1640 470 ml + Gent (1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) Procedure Draw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml
Blastocyst embryo transfer into pseudopregnant recipient female mouse(图)
. The Recipient Careful selection of the recipient is most important as the pups are the end result of a lot of hard work. I personally use Swiss Webster or CD-1 mice, as they are quiet and make excellent mothers, although do become overweight quickly
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