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文献和实验Solutions for Tissue/Cell Digestion
Solutions for Tissue/Cell Digestion Trypsin (13 ug/ml) in Saline G Materials Worthington 3x crystallized trypsin (LS03707), 1.3 mg. Sterile Saline G (pH 7.0), 100 ml. Procedure (all steps on ice) 1. Dissolve
Myosin Light Chain Preparation
Buffer A.Homogenizing Buffer: 28 liters: 50 mM KCl,20 mM Tris (pH 8.0),15 mM MCE, 0.2 mM MgAc2 (350 ml 4 M KCl,280 ml 2 M Tris,28 ml 15 M MCE,1.20 g MgAc2) Buffer B.Triton X-100 Buffer: 4 liters of Buffer A plus 40 ml X-100 (1% Triton final) Buffer C
Purification of Calmodulin from Bovine Brain
ml DE-52 column equilibrated with 50 mM MOPS,pH 7.0,0.1 M KCl. 2.Centrifuge the dialyzed sample at 50,000 x g for 30 min and apply supernatant fraction to equilibrated column. 3.Wash the column with 50 mM MOPS,pH 7.0,0.1 M KCl until no more protein
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