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文献和实验Multiplex PCR: Critical Parameters and Step-by-Step Protocol
. Based on this experience, a step-by-step multiplex PCR protocol was designed (Figure 1), with practical solutions to many of the problems encountered. This protocol should be useful to those using PCR technology in both the research and the clinical
One step method for the isolation of plasmid DNA
, they are therefore cumbersome, time consuming and not economical. The technical report below by Chowdhury, K. (1991) is a very fast, simple and one step ''miniprep'' procedure. The quality and quantity of DNA obtained by using this procedure is similar to those obtained
Multiplex PCR: Critical Parameters and Step
and 5e. Genomic DNA was prepared using a standard sodium dodecyl sulfate (SDS)/proteinase K protocol (Boehringer Mannheim). 全文下载:Multiplex PCR: Critical Parameters and Step-by-Step Protocol
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