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文献和实验-mediated genome engineering. Cell 154(6):1370-1379. doi: 10.1016/j. cell.2013.08.022 25. DiCarlo JE, Norville JE, Mali P, Rios X, AachJ, Church GM (2013) Genome engineering inSaccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res 41
analysis of genomic DNA by southern blotting
reaction (10 ng/µl) 5 x *dATP buffer (for use with [a -32 P]dATP) containing dGTP, dCTP, dTTP, or 5 x *dCTP buffer (for use with [a -32 P]dCTP) containing dGTP, dATP, dTTP [a -32 P]dATP) or [a -32 P]dCTP) - not provided by the kit
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Transfection Kit (Clontech) CD34+ cell separation kit (CD34 MicroBead Kit containing anti-human CD34 MicroBeads and Blocking Reagent; Miltenyi Biotec) Cellgro medium (serum-free medium; Cellgenix) DMEM for HSC Fetal calf serum (FCS
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