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文献和实验a stir bar and ice pack, and start transfer 7. Time and voltage of transfer will vary depending on the properties of the protein and the transblot* buffer used. Continuous Semidry Transfer: 1. Disassemble gel-casting plates
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
) (see Note 5 ). 5. Rabbit anti-mouse (#31450), sheep anti-rabbit (#31460)and donkey anti-sheep (#31480) horseradish peroxidaseconjugatedsecondary antibodies are available from Pierce(Rockford, IL). 6. Enhanced chemiluminescence (ECL) reagent (#RPN2106
Synthesis and Probing of Membrane-bound Peptide Arrays
DMF This should be free of contaminating amines and thus of the highest affordable purity. Purity can be checked by adding 10 µl of BPB stock to 1 ml of DMF. If the resulting color is yellow, the batch
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