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文献和实验Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
- phenol reagent B : Add l00g of Na 2 WO 4 • 2H 2 O, 25g of Na 2 MoO 4 • 2H 2 O, 700ml of distilled water, 50ml of 85% H 3 PO 4 and 100ml of thick HC1 into a 1.5L round- bottom stoppered flask, mix
standard dilutions of BSA of 25, 50, 75 and 100 µ g/ml. Prepare appropriate serial dilutions of the sample to be measured. Place 1.0 ml of each of the above into separate tubes. Add 100 µ l of sodium deoxycholate to each tube.
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