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货号:2050-0021
产品名称:PAL ASSY,1000S U13
品牌:abi
关键字:abi,Applied Biosystems公司,2050-0021,PAL ASSY,1000S U13
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文献和实验PCR PRIMER DESIGN AND REACTION OPTIMISATION
of the Tm is Tm = 4(G + C) + 2(A + T)o C. Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s). One should aim at using an annealing temperature (Ta) about 5o C below the lowest Tm of ther pair
., Preuss, D., Leinonen, R., Shumway, M., Sherry, S., Flicek, P.; 1000 Genomes Project Consortium. 2012. The 1000 Genomes Project: Data management and community access. Nat. Methods 95:459‐462.
Biennial Conference, Pal Beach, Florida, September 1994, vol. I 149-163. 9.Y. Ayash, H. Imai, T. Yamada, T. Fakuda, Y. Yanaga and T. Taniyama. Performance of reverse osmosis membranes in Jeddah Phase I plant. Desalination, 96(1994) 215-224. 10.S
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