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文献和实验Maltose-Binding Protein as a Solubility Enhancer
as a general solubilizing agent, this has not turned out to be the case. In a direct comparison with glutathione S-transferase (GST) and thioredoxin, maltose-binding protein (MBP) was decidedly superior at solubilizing a diverse collection of aggregation-prone
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Table I Species Protein A Protein G Human ++++ ++++ Mouse ++ ++ Rat
Purification of MBP (maLTose-binding proteins) Fused Proteins
with 10 fractions of 10 mM maltose in 20 mM Tris pH 7.4, 200 mM NaCl, 10 mM b ME, 1 mM EDTA or in some other buffer of choice, depending on subsequent purification steps. If the fusion protein is to be cleaved with factor Xa as the next step
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