- ￥8579
- Cell Applicatins
- 026-500
- 美国
- 2026年03月20日
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孚博生物
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500 ml
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文献和实验Culture of BEND Cells (Bovine Endometrial Cells)
Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse serum (H1138) Antibiotic-antimycotic (ABAM) (A9909) D-valine (V1255) Insulin (I5500) Preparation 1. Place 1000 ml of double distilled water
Culture of BEND Cells (Bovine Endometrial Cells)
CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma ; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse
Procedure for Transfection of Mammalian Cells
IMDM containing 20% fetal bovine serum, 1% glutamine, 1% aa In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml IMDM containing 10% FBS and nonessential amino acids. Incubate the cells at 37E C
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