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文献和实验E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
The E2F-family of transcripion factors exerts fascinating and contrasting functions in tran-scriptional repression and activation of genes regulating proliferation, apoptosis, and differentiation. E2F is principally regulated by its temporal
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
The tetracycline-regulated expression system developed by Gossen and Bujard is a powerful genetic tool that permits the expression of any gene construct introduced into either cultured cells or transgenic animals to be precisely controlled
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