- ￥2800
- 戴格生物
- 中国
- PKT377D-PBS
- 2026年03月20日
- ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
IgG
- 保存条件：
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
- 克隆性：
Monoclonal
- 标记物：
Un-conjugated
- 适应物种：
Human
- 供应商：
杭州戴格生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA
- 浓度：
2 mg/ml
- 靶点：
TNF beta
- 抗体名：
TNF beta抗体对
- 规格：
100μg
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文献和实验Dual- and Triple-Color Fluorospot
100 μl/well of the mixed mAb solution and incubate overnight at 4–8°C (seeNote 1). c. After coating, empty the plate and wash five times with sterile PBS (200 μl/well) to remove excess mAb. Block the plate for at least 30 min with 200 μl
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
Size‐Exclusion Chromatography with On‐Line Light Scattering
Figure 20.6.5 Chromatograms of TNF, sTNFR, and the mixture of sTNFR and TNF. 100 µl of each protein was injected onto a Superose 12 column (Pharmacia) with PBS as eluent at a flow rate of 0.5 ml/min. (A ) TNF
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