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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
IgG
- 保存条件:
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
- 克隆性:
Monoclonal
- 标记物:
Un-conjugated
- 适应物种:
Human
- 供应商:
杭州戴格生物技术有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
2 mg/ml
- 靶点:
Antithrombin III/ATIII
- 抗体名:
Antithrombin III/ATIII抗体对
- 规格:
100μg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
dilutions for rabbit polyclonal antibodies range between 1:50 and 1:500. For double-labeling experiments, use primary antibodies raised in two different species. Antibodies, secondary Secondary antibodies are diluted in PBS containing 2% normal
操作步骤参考[3] 1. 收集:100g 离心 5min 收集类器官。 2. 重悬:收集到的类器官重悬于 1× PBS 中,转移至 96 孔圆底 (U 型) 板中,100g 离心 5min。 3. 固定:弃上清,加入 4% 多聚甲醛室温固定 30min。 4. 洗涤:移除多聚甲醛,用 1× PBS 洗涤 3 次,每次 100g 离心 5min。 5. 透化:加入 0.3% Triton-X,室温透化 10min。 6. 封闭:加入含有 10% 驴血清的 PBST
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
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