Purified recombinant protein o

Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions

Basic Protocol 1: Production of Extracellular Protein Microarrays   Materials Purified proteins

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

in solution is a monomer, dimer, or oligomer, or if it forms a complex with other proteins. If one has a purified protein, the molecular weight can be determined quite accurately by sedimentation equilibrium in the analytical ultracentrifuge. This technique

（共享）蛋白分析方法之免疫沉淀

of an insoluble antibody-antigen complex. Rather, antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody (Figure 1). Thus, unlike other techniques based