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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse ASH2 like histone lysine methyltransferase complex subunit (Ash2l), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验of the basic nucleosome model of chromosome structure, various methods have been developed for mapping and characterization of histone and non-histone protein-DNA interactions. Most often, enzymatic (e.g., micrococcal nuclease, DNase I, DNase II, restriction
Installation of Site‐Specific Methylation into Histones Using Methyl Lysine Analogs
of analogs of methyl lysine residues into recombinant histones. The modification site is specified by mutating the lysine of interest to cysteine. The mutant histones are expressed and purified, and the cysteine residue alkylated to produce N?methyl
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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