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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse tetraspanin 12 (Tspan12), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
METHOD Protocol 1: Preparation of OP9 Cells for Co-culture Thawing OP9 or OP9-DL1 Cells 1. Add 12 mL of OP9 medium to a 15-mL centrifuge tube. 2. Thaw OP9 or OP9-DL1 cells quickly in a 37°C water bath.
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
multiplerounds of freeze–thawing. Pre-incubation of Wnt samples with sFRP protein for 30 min at 37°C is sufficientto inhibit Wnt activity in various assays. The concentration of sFRP required to block Wnt signalingshould be determined empirically, as it will vary
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