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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse small nuclear ribonucleoprotein 35 (U11/U12) (Snrnp35), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验Yeast Nuclear Extract (Small Scale)
)containing DTT and protease inhibitors.Depending on protein pellet size,resuspend in 1.5 - 0.4 ml buffer.This can be done with a small dounce homogenizer or a blue pipette tip depending on the amount of protein. Dialyze nuclear extract vs Buffer C + 75 mM
K-U4atac] small nuclear ribonucleoprotein (snRNP) particles prepared by reconstitution in vitro and U1 snRNP particles purified from HeLa cells.
Yeast Nuclear Extract (Small Scale)
tubes and pellet crude nuclei.Spin 13 K rpm for 30 min.in SS34 rotor.Remove supernatant by dumping and discard.Drain pellets. Resuspend crude nuclear pellets with a small spatula in 10 ml Buffer B and transfer to 50 ml screwcap tubes.The prep
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