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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 保质期:
见瓶身
- 英文名:
Mouse IgD Monoclonal Antibody (11-26c (11-26)), APC, eBioscience™
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
/
- 规格:
25μg
产品详细信息
Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
using the ClonaCell™ -HY Hybridoma Cloning Kit. Six hybridoma clones secreting Blo t 11 mAb were successfully generated, and these mAb are useful reagents for immunoaffinity purification and immunoassays.
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
at the 3’ end from Sigma-Aldrich.The (1-mercaptoundec-11-yl)tetra(ethylene glycol) (MUTEG) was purchased from Sigma-Aldrich. Immobilization of the human IgE (hIgE) aptamer on the SPRi-Biochip™ The thiol modified IgE aptamer and reference
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