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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见说明书
- 保质期:
见说明书
- 英文名:
Recombinant Saccharomyces cerevisiae Protein ICE2(ICE2),partial
- 库存:
99
- 供应商:
武汉华美生物/CUSABIO
- 规格:
20ug/100ug/1mg
ICE2蛋白|Recombinant Saccharomyces cerevisiae Protein ICE2(ICE2),partial|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID:P40499
别名:ICE2; YIL090WProtein ICE2; Inheritance of cortical ER protein 2
种属:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
氨基酸序列:参见官网
微信ID: Cusabio01 电话: 027-87939808
详情请登录:www.cusabio.cn
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文献和实验Yeast Protein Isolation "Yaffe-Schatz"
1.Grow 25mls yeast cells to 5x10E6. 2.Sequentially spin down cells in a 15 ml polypro tube. 3.Wash cells with 1ml ice cold ddH2O,transferrring to an eppy tube. 4.Recon.cells in 1 ml ice cold ddH2O containing 100μg/ml PMSF. 5.Add 150μl ice cold
Yeast Protein Isolation Yaffe-Schatz
Yeast Protein Isolation "Yaffe-Schatz" David Amberg Adapted from Yaffe, M.P. and Schatz, G., PNAS, 1984, 81, 4819-4823. Grow 25mls yeast cells to 5x10E6. Sequentially spin down cells in a 15 ml polypro tube. Wash
Preparation of Yeast Protein Extracts
Preparation of Yeast Protein Extracts (see Clontech YPH, p12) Cracking buffer stock solution (see p54 Clontech YPH) 8M Urea; 5% w/v SDS; 40mM Tris-HCl pH6.8; 0.1mM EDTA; 0.4 mg/ml BMB in H
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