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文献和实验. These charged acids can be removed by passage through a mixed-bed resin (e.g. Bio-Rad AG 501-X8). This must be done quickly, minimizing exposure of the glyoxal to air, or it will just reoxidize as you work! Our method is to use a baked spatula to load
LABELING ALPHA-ACTININ WITH IATR
Materials 1.Small vial and stir bar. 2.Bio-Beads SM-2 in 0.7x15 cm column. 3.2 mM Tris-HCl,pH 8.5,4℃,250 ml. 4.Centricon-30 concentrator. 5.5 mM Tris-acetate,0.1 mM DTT,pH 6.95,4℃,250 ml. 6.200 mM K-borate,pH 9.0,10 ml. 7.DTT,100 mM stock
(e.g. Bio-Rad AG 501-X8). This must be done quickly, minimizing exposure of the glyoxal to air, or it will just reoxidize as you work! Our method is to use a baked spatula to load about 5 mls of resin into each of ~3 small Bio-Rad dispo-columns
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