HT-W330G轨道摇床

拟南芥叶绿体蛋白质组学分析

8.0) 、5 mmol/L MgCl2、蛋白酶混合抑制剂（表 6-1)。 ( 4 ) 100% PF-Percoll：0.8 g PEG 8000、0.27 g Ficoll  400000，加 Percoll 至最终体积为 27.5 ml。 ( 5 ) Percoll 梯度：PF Percoll  ( 40% 或 85% ) 、0.5 mmol/L EDTA、50 mmol/L HEPES-KOH ( pH 8.0) 、330 mmol/L sorbitol。 ( 6 ) 浓缩

关于如何计算引物和核酸浓度的方法

a) Primers: i) Oligonucleotide primers are generally supplied as "so many OD units/ml" - but what does this mean, in terms of mg/ml, or mmol/ml, etc? Given: a primer is Y nucleotides (nt) long; Given: the MW of ssDNA is (330 daltons per nt) x

Calculating Concentrations for PCR

X1000/330Y uM For example: B 88/77 primer - a 17-mer oligodeoxynucleotide - as supplied is 12.6 OD units/ml. We need to make a 5 uM stock solution for PCR.MW: 17 x 330 = 5610 Concentration: 12.6 OD x 37 ug/ml = 466 ug/ml = 466 mg/l = 0.466 g/l