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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Neosartorya fumigata Asp f 15 protein
- 形态:
liquid
- 保存条件:
见COA
- 克隆性:
多克隆
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 应用范围:
ELISA, IHC, WB
- 抗体英文名:
Anti-Neosartorya fumigata Asp f 15 Polyclonal Antibody
- 抗体名:
抗Neosartorya fumigata Asp f 15多抗
- 规格:
100ul; 500ul; 1ml

| 产品信息
品名:NebuSelect™ Anti-Neosartorya fumigata Asp f 15 Polyclonal Antibody
别名:Allergen Asp f 15, Allergen Asp f 13, Asp f 15, AFUA_2G12630
货号:NBAB-014854
品牌:Nebulabio
规格:100ul; 500ul; 1ml
| 产品描述
NebuSelect™ Anti-Neosartorya fumigata Asp f 15 Polyclonal Antibody (Cat# NBAB-014854) was produced in rabbits immunized with recombinant Neosartorya fumigata Asp f 15 protein, and it reacts with Neosartorya fumigata (Aspergillus fumigatus). This antibody is validated for ELISA, IHC, WB.
| 产品属性
Product Category:Rabbit PAb
Reactivity:Neosartorya fumigata (Aspergillus fumigatus)
Specificity:Neosartorya fumigata Asp f 15
Immunogen:Recombinant Neosartorya fumigata Asp f 15 protein
Isotype:Polyclonal Rabbit IgG
Purification:Protein A & Protein G Affinity Purification
Buffer:0.2 μm filtered solution in PBS, pH7.4, containing 0.09% Sodium azide, 50% glycerol.
Conjugate:Unconjugated
Applications:ELISA, IHC, WB
Dilution:ELISA 1:2000~1:10000; IHC-P 1:50~1:500; WB: 1:1000~1:5000
Storage:This liquid antibody can be stored at 2℃-8℃ for at least two weeks. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or antibody diluent, the antibody is stable for at least two weeks at 2-4℃.
For Research Use Only!
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文献和实验),18%(8/45)。 2.2 用补充的Chubick法判断,45例SLE病人中有27例处于活动期,抗CyP A自身抗体阳性率为63%(17/27),与非活动组(28%,5/18)差别显著(P<0.05)。其中重度活动组有12例,抗体阳性率75%(9/12),与非重度活动组(53%,8/15)相比无统计学差异(P>0.05)。SLE病人各项临床表现与抗CyP A抗体的关系见表1。 表1 SLE病人临床表现与抗CyP A抗体的关系
DNA聚合酶,限制性内切酶mluI,nheI,sphI,notI均购自GIBCOBRL公司,抗CD3单克隆抗体HIT3a由本所协和科技公司提供,抗Pgp单克隆抗体PHMA02由本室制备。鼠抗E-tag抗体,HRP标记的鼠抗E-tag抗体及抗E-tag亲和层析柱均购自Pharmacia公司。FITC标记的羊抗鼠IgG多抗购自北京中山生物工程公司。其它均为国产分析纯生化试剂。(3)PCR引物P1 5’ CTA GCG CAC GCG TAC GCT GAC ATC GAG CTC ACT CAG TCT
液洗膜几秒种。 5. 将膜置于封闭液中,室温下封闭30 min。 6. 准备抗体溶液:用封闭液按1∶5000的比例稀释Anti-DIG-AP。 7. 将膜置于抗体溶液中,室温下保持30 min,注意抗体溶液必须没过膜。 8. 室温下用洗膜缓冲液洗膜2次,每次15 min。 9. 再将膜置于检测缓冲液中2 min。 10. 在10 ml 检测缓冲液中加入45 μl NBT和35 μl BCIP配制成显色底物溶液(现配现用)。 11. 除去检测缓冲液,加入显色
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