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文献和实验Flow Cytometry of Fibroblast Nuclei for DNA content
in 0.5 mL PBS. Remove 0.1 mL for flow cytometry and use the remainder for protein extracts. Add 4 - 5 volumes of 100% EtOH and vortex gently. Incubate 15'. (The cells may be now stored at 4ºC.) Spin at 1000 RPM, and wash pellet with PBS.
ul propidium iodide prior to analysis to detect dead cells (optional) Calibrate the FACScan by standard fluorescent beads (optional)Flow Cytometry Analysis Flow cytometers are complex instruments that require a well-trained operator. Prior to use
Analyzing p53 Regulated DNA Damage Checkpoints by Flow Cytometry
amount of DNA. Flow cytometry is a standard technique that is used to ‘sort’ cells based on their DNA content. It uses the principles of light scattering, light excitation, and emission of fluorochrome molecules to generate data about individual cells. The cells
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