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- CTNI-7-HS
- 2026年03月16日
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文献和实验ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig and to mouse IgG1 and IgG3 . Protein G can also bind bovine serum albumin (BSA). Thus, BSA should be added to buffers used with Protein G. Alternatively, recombinant Protein G
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
should cover it. Incubate at RT for 1 hr or longer, depending on the antibody. Sometimes an overnight inc ubation is helpful. Jon's anti-actin Guinea pig antibodies from animal#1 are best at 1:6,000, #2 and #3 are best at 1:2,000. Wash the cells 4 x
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