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文献和实验Clonality - X Chromosome Inactivation Assay
sample under study is ideal. D: PCR Set up the following PCR reaction: Cycles are as follows: E: Gel Electrophoresis Prepare gel consisting of 6% acrylamide as in LOH protocol. Add 4 µl dye to 20 µl PCR product. Denature the samples
Transformation of E. coli by Electroporation
100% ethanol 70% ethanol 0.5X TE SOC medium transformation plates I. Preparation of E. coli cells
Transformation of E. coli by Electroporation
:100 dilution of the cell suspension. Dilute the cell suspension to a concentration of 2 x 1010 to 3 x 1010 cells/ml (1.0 OD600 = approx. 2.5 x 108 cells/ml) with ice-cold GYT medium. 9. Transfer 40 μl of the suspension to an ice-cold
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