- ￥3996
- Abcam
- ab46543
- 2026年03月15日
- Gt
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
4 Hydroxynonenal
- 宿主：
Gt
- 保存条件：
4°C/-20°C
- 亚型：
IgG
- 规格：
100ul
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文献和实验Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
. Before stopping the reaction, check the nick-translation reaction by running 1/10 of the reaction on a 1% agarose gel. One should obtain a smear of biotin labeled fragments ranging from 500 to 200 nt. Stop the reaction according manufacturer instructions.
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