- ￥3996
- Abcam
- ab76871
- 2026年03月15日
- Ms
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HLA G
- 宿主：
Ms
- 规格：
100ug
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文献和实验Polyclonal and Monoclonal Antibodies
1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate
Purification of Endotoxin Free mAbs
from ascitic fluid preparations). To make a 10x stock of buffer A: Malonic Acid 104g NaOH 60g Betaine 20g Make to 2 litres with endotoxin free water
Western Blotting with Monoclonal Ant
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. Polyacrylamide Gel
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