- 询价
- Invitrogen
- 美国
- 333500
- 2026年03月14日
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- 供应商：
南京赛泓瑞
- 规格：
100 UG
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µ l. Incubate for 4 hours at 4°C Wash once with cold cyclin staining buffer and once with cold PBS/azide. Resuspend the cells in propidium iodide at 50 µ g/ml
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
the need for fluorescence compensation. This assay can be used with any instrument employing dual laser excitation, including the B-D Vantage, FACSCalibur and Coulter Elite. Materials Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326) or cyclin
Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. The gene encoding the cyclin D1 protein, a regulator of the progression from the G1- to S-phase, is often found disrupted in the cancer cell
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