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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 英文名:
Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified
- CAS号:
/
- 保质期:
见瓶身
- 供应商:
麦格生物
- 保存条件:
−20°C
- 规格:
5mL
Geltrex LDEV-free stem cell-qualified reduced-growth factor basement membrane matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV) sometimes found in this type of preparation. Routinely used for attachment and maintenance of human iPSCs or embryonic stem cells, every lot of this matrix is quality control tested using hESCs or iPSCs to help ensure growth and pluripotency of each lot.
Features of Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, include:
Every lot qualified using hESC or iPSC, helping reduce the need for customers to lot screen
Free of lactose dehydrogenase elevating virus (LDEV), making it safe for all types of cell culture
Consistent protein concentration lot-to-lot, helping ensure reproducible results
Gain reliability and consistency in your hESC/iPSC studies
Human hESCs/iPSCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, has been tested for support of hESC/iPSC growth and pluripotency, minimizing the need to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, so you can have confidence in every purchase.
Free of LDEV contamination
Recent advances in processing of EHS tumor cells have resulted in virus-free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can be confident that every lot is virus-free.
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文献和实验Binding Models of Copper(II) Thiosemicarbazone Complexes with Human Serum Albumin: A Speciation Study.
Nóra V May et al.
Molecules (Basel, Switzerland), 26(9) (2021-06-03)
Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
实验步骤 There are many applications for Basement Membrane Matrix which require different thicknesses and concentrations. In general, a protein concentration > 9 mg/ml, is used for differentiation studies of primary cells
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
from its endogenous mesenchyme, undergoes branching morphogenesis when cultured ex vivo in a basement membrane extract in serum-free medium with growth factor stimulation. The resulting three-dimensional epithelial morphogenesis in the defined culture system makes
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