pLV3-CMV-TRIM32-3×FLAG-CopGFP-

pLV3-CMV-TRIM32-3×FLAG-CopGFP-Puro plasmid

PVT112122

pLV3-CMV-TRIM32-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TRIM32(human)-3×FLAG-CopGFP-Puro
Alias: NM_012210.4;BBS11; HT2A; LGMD2H; LGMDR8; TATIP
Gene lenght: 1959
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM32-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TRIM32-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TRIM32-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctgcagcagc agcttctcac 2341 ctgaacctgg atgccctccg ggaagtgcta gaatgcccca tctgcatgga gtccttcaca 2401 gaagagcagc tgcgtcccaa gcttctgcac tgtggccata ccatctgccg ccagtgcctg 2461 gagaagctat tggccagtag catcaatggt gtccgctgtc ccttttgcag caagattacc 2521 cgcataacca gcttgaccca gctgacagac aatctgacag tgctaaagat cattgataca 2581 gctgggctca gcgaggctgt ggggctgctc atgtgtcggt cctgtgggcg gcgtctgccc 2641 cggcaattct gccggagctg tggtttggtg ttatgtgagc cctgccggga ggcagaccat 2701 cagcctcctg gccactgtac actccctgtc aaagaagcag ctgaggagcg gcgtcgggac 2761 tttggagaga agttaactcg tctgcgggaa cttatggggg agctgcagcg gcggaaggca 2821 gccttggaag gtgtctccaa ggaccttcag gcaaggtata aagcagttct ccaggagtat 2881 gggcatgagg agcgcagggt ccaggatgag ctggctcgct ctcggaagtt cttcacaggc 2941 tctttggctg aagttgagaa gtccaatagt caagtggtag aggagcagag ttacctgctt 3001 aacattgcag aggtgcaggc tgtgtctcgc tgtgactact tcctggccaa gatcaagcag 3061 gcagatgtag cactactgga ggagacagct gatgaggagg agccagagct cactgccagc 3121 ttgcctcggg agctcaccct gcaagatgtg gagctcctta aggtaggtca tgttggcccc 3181 ctccaaattg gacaagctgt taagaagccc cggacagtta acgtggaaga ttcctgggcc 3241 atggaggcca cagcgtctgc tgcctctacc tctgttactt ttagagagat ggacatgagc 3301 ccggaggaag tggttgccag ccctagggcc tcacctgcta aacagcgggg tcctgaggca 3361 gcctccaata tccagcagtg cctctttctc aagaagatgg gggccaaagg cagcactcca 3421 ggaatgttca atcttccagt cagtctctac gtgaccagtc aaggtgaagt actagtcgct 3481 gaccgtggta actatcgtat acaagtcttt acccgcaaag gctttttgaa ggaaatccgc 3541 cgcagcccca gtggcattga tagctttgtg ctaagcttcc ttggggcaga tctacccaac 3601 ctcactcctc tctcagtggc aatgaactgc caggggctga ttggtgtgac tgacagctat 3661 gataactccc tcaaggtata taccttggat ggccactgcg tggcctgtca caggagccag 3721 ctgagcaaac catggggtat cacagccttg ccatctggcc agtttgtagt aaccgatgtg 3781 gaaggtggaa agctttggtg tttcacagtt gatcgaggat caggggtggt caaatacagc 3841 tgcctatgta gtgctgtgcg gcccaaattt gtcacctgtg atgctgaggg caccgtctac 3901 ttcacccagg gcttaggcct caatctggag aatcggcaga atgagcacca cctggagggt 3961 ggcttttcca ttggctctgt aggccctgat gggcagctgg gtcgccagat tagccacttc 4021 ttctcggaga atgaggattt ccgctgcatt gctggcatgt gtgtggatgc tcgtggtgat 4081 ctcatcgtgg ctgacagtag tcgcaaggaa attctccatt ttcctaaggg tgggggctat 4141 agtgtcctta ttcgagaggg acttacctgt ccggtgggca tagccctaac tcctaagggg 4201 cagctgctgg tcttggactg ttgggatcat tgcatcaaga tctacagcta ccatctgaga 4261 agatattcca ccccaggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 4321 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 4381 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4441 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4501 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4561 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4621 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4681 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4741 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4801 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4861 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4921 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4981 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 5041 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 5101 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 5161 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 5221 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 5281 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 5341 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 5401 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5461 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5521 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5581 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5641 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5701 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5761 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5821 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5881 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5941 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 6001 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 6061 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6121 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6181 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6241 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6301 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 6361 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6421 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6481 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6541 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6601 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6661 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6721 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6781 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6841 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6901 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6961 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 7021 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 7081 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7141 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7201 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7261 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7441 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7561 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7621 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7681 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7741 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8701 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8761 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8821 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8881 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8941 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9001 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9061 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9121 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9181 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9241 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9301 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9361 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9421 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9481 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9541 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9601 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9661 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9721 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9781 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9841 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9901 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9961 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 10021 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 10081 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 10141 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 10201 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.