pLV2-CMV-GTF3C2-3×FLAG-Puro pl

pLV2-CMV-GTF3C2-3×FLAG-Puro plasmid

PVT112037

pLV2-CMV-GTF3C2-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-GTF3C2(human)-3×FLAG-Puro
Alias: NM_001035521.3;TF3C-beta; TFIIIC-BETA; TFIIIC110
Gene lenght: 2733
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-GTF3C2-3×FLAG-Puro plasmid Description

pLV2-CMV-GTF3C2-3×FLAG-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-GTF3C2-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggatac ctgcggggtc ggctatgttg ccctggggga 2881 ggccggcccc gtggggaaca tgactgtggt agactctcct ggacaagagg tgctaaatca 2941 gcttgatgtc aagacctctt cagaaatgac cagtgcagag gcttccgtag agatgtcatt 3001 acctacccct ttgcctggat ttgaggattc tcctgatcag aggaggctcc ctccagagca 3061 ggaaagcctc tccagactgg aacagccaga tctttcttca gagatgtcaa aggtctcaaa 3121 gcctagggcc tcaaagcctg gccggaagag aggtggtagg acacgaaaag gccccaaaag 3181 gccccaacag cctaatcctc catcagcccc actggttcct ggtctcttag atcaatccaa 3241 ccctctgtcc acccccatgc ctaagaaacg aggtcgaaag tccaaggcag agctgctgct 3301 gctgaagttg tcaaaagacc tagatcggcc agaatctcaa tctccaaaga ggccccctga 3361 ggactttgag accccttctg gggaacgacc ccgccgaagg gctgcccaag tggcacttct 3421 gtatcttcag gaactggctg aagagctctc aacagccctg cctgcccctg tgtcctgtcc 3481 tgagggcccc aaggtgagca gccccaccaa accgaagaag atccggcagc cagcagcctg 3541 tccaggtgga gaagaggtgg atggtgctcc acgggatgaa gacttttttc tccaggttga 3601 ggctgaagat gtggaagaaa gtgagggccc aagtgagagc tcatctgaac ctgagcctgt 3661 agtgccccga agcaccccac gaggatctac ttcagggaaa cagaaaccac actgccgagg 3721 aatggctccc aatggcttac caaatcatat catggctcct gtttggaagt gcctccatct 3781 caccaaggac ttccgagagc agaaacattc atactgggag tttgctgagt ggattccttt 3841 agcctggaag tggcacttgt tatctgagct tgaggccgct ccctacctgc cccaggagga 3901 gaagtctcca ttgttttctg tacaacgtga agggctacct gaagatggca ccctctaccg 3961 aataaacaga tttagctcga tcacagcaca tccagagcgc tgggatgtgt ccttcttcac 4021 ggggggaccg ctctgggctc tggactggtg cccagtgcca gagggggcag gagcctcgca 4081 atatgtggct cttttctcca gccctgacat gaatgagaca cacccactga gccagcttca 4141 ttcgggtcct gggctgctcc agctctgggg ccttgggacc ttgcagcaag aaagctgtcc 4201 tggcaacagg gcccactttg tctatgggat tgcttgtgac aacggctgca tctgggacct 4261 caagttctgc cccagtggag catgggaact tccaggcacc cctcggaagg ctcctctcct 4321 gccccggttg ggtctcttgg ctctggcctg ctcagacggg aaagtactgc tattcagtct 4381 accccatccg gaggccctgc tggctcagca acccccagat gcagtgaagc ctgccatata 4441 taaggtacaa tgtgtggcaa ctctgcaggt ggggtctatg caagctacag acccctctga 4501 gtgtggtcag tgccttagcc tggcctggat gcctaccagg ccccaccaac acctagctgc 4561 tggatattat aatggcatgg tggttttctg gaaccttccc actaactcac ccctgcagcg 4621 gatacggctc tctgatggct ccttaaagct ctaccccttc cagtgtttcc tagcccatga 4681 ccaggctgtg cgtacccttc aatggtgcaa agctaacagc catttccttg tctctgcggg 4741 gagtgaccgg aaaatcaaat tctgggacct tcgacgtcct tacgaaccca taaactctat 4801 caagcgcttc ttgagtacag aactggcctg gctgcttccc tacaatggtg tcactgtggc 4861 tcaggacaac tgctatgcct cttatggact ctgtgggatt cattatattg acgctggtta 4921 ccttggtttc aaggcctact tcactgctcc tcgaaaaggc accgtttgga gtctttcagg 4981 atccgactgg cttgggacaa tagctgcagg agatatatcc ggggagctca ttgctgctat 5041 attaccagat atggcactga atccaataaa tgtcaagcga cctgtagagc gaagatttcc 5101 tatatataaa gcagatctga taccgtatca ggacagtcct gaaggtccag accattcttc 5161 tgcttcatct ggggtcccca accctcctaa ggctcgaact tacactgaaa ctgtcaacca 5221 tcactacttg ctctttcaag acacagattt gggttcattc catgatctgc tccgtagaga 5281 accaatgctg cgcatgcagg agggagaggg gcattctcaa ctctgcctgg acaggctgca 5341 gctggaggct attcataagg tacgtttcag cccaaacctg gactcctatg gatggctggt 5401 atctgggggg cagtcagggc tggttcgaat ccattttgtc cgtggactcg cctccccact 5461 gggccaccgt atgcagcttg aaagccgagc ccacttcaat gctatgttcc aaccatcctc 5521 ccccactaga cggcctggct tctctccaac cagccatcgc cttctgccca ctcccggatc 5581 cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA 5641 TGACAAGtag tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 5701 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 5761 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 5821 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 5881 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 5941 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 6001 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 6061 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 6121 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag 6181 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 6241 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 6301 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 6361 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 6421 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 6481 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 6541 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 6601 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 6661 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 6721 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 6781 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 6841 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 6901 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 6961 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 7021 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 7081 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 7141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 7201 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 7261 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 7321 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 7381 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 7441 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 7501 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 7561 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga 7621 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag 7681 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 7741 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta 7801 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 7861 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac 7921 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc 7981 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc 8041 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 8101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 8161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 8221 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 8281 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga 8341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 8401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 8461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 8521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 8581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 8641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 8701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 8821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 8881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 9001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 9061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 9121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 9181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 9241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 10441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 10501 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac 10561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 10621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 10681 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 10741 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 10801 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 10861 gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.