pAAV-CMV-mCherry-U6-Ogn-shRNA1

pAAV-CMV-mCherry-U6-Ogn-shRNA1 plasmid

PVT112031

pAAV-CMV-mCherry-U6-Ogn-shRNA1 plasmid Information

Plasmid name: pAAV-CMV-mCherry-U6-Ogn(mouse)-shRNA1
Alias: CGTGTAATTCACCTTCAGTTT
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Gene interference
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: mcherry-F
Reverse primer:
Copy number: High
Induction:

pAAV-CMV-mCherry-U6-Ogn-shRNA1 plasmid Description

pAAV-CMV-mCherry-U6-Ogn-shRNA1 This plasmid is suitable for gene interference in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CMV-mCherry-U6-Ogn-shRNA1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGcCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCGCTAGCGT TTAAACTTAA GCTTGGTACC ACTAGTGATA TCTCTAGAGG 1381 GCCCAGATCT ACgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 1441 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 1501 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 1561 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 1621 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 1681 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 1741 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 1801 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 1861 cctgtccttc tgattttgta ggtaaccacg tgcggaccTC GAAATTCCCC AGTGGAAAGA 1921 CGCGCAGGCA AAACGCACCA CGTGACGGAG CGTGACCGCG CGCCGAGCGC GCGCCAAGGT 1981 CGGGCAGGAA GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC 2041 TGTTAGAGAG ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC 2101 GTGACGTAGA AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT 2161 GGACTATCAT ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGGTT TATATATCTT 2221 GTGGAAAGGA CGCGGGATCC GCGTGTAATT CACCTTCAGT TTctcgagAA ACTGAAGGTG 2281 AATTACACGt tttttCTCGA GTTAGAGTCC TGCAGCTCGC TTTTTAAGCT TGCGGccgca 2341 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 2401 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 2461 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg 2521 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc 2581 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2641 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2701 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2761 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2821 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 2881 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 2941 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 3001 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 3061 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 3121 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 3181 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 3241 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 3301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 3361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 3421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 3481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 3541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 3601 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 3661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 3841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 3901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 3961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 4021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 4081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 4141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 4201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 4261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 4321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4561 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 5041 cggttcctgg ccttttgctg gccttttgct cacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.