pNEK10-Fluc-SV40-hRluc-Neo pla

pNEK10-Fluc-SV40-hRluc-Neo plasmid

PVT111971

pNEK10-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pNEK10(human)-Fluc-SV40-hRluc-Neo
Alias:
Gene lenght: 2801
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: NEK10
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pNEK10-Fluc-SV40-hRluc-Neo plasmid Description

pNEK10-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pNEK10-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcT TTGAAAGCTG AATGGATAGT GATGCCCAAA ATAGGGAATC CAAGAGCAGC 5161 AGCAGACATG GGGAAGATGG CGTTCAGGGT TGCACATGTG TGGCGTTAAC TTGGCAGCTG 5221 GATTTTGGGG TCTGACAGTT AGTAGAGCAG GCTAGGTTCC AGTTGGAGAC TTTCTAATCA 5281 TCGTCAGATA GATGGTGCCT GAGGCGAGGA AGTGGAAGGG CTCACCGAAG TAGGGCAGAA 5341 AAGCAGAGGG GCAAAGTCAA AGCAGCATCC TGTAAGGAGA AGGCAGAAAA GCGACGCTCA 5401 TAGAGATGAA GAATGAACCT CCAGAGGCGT CGGAGCAGAT TGGAAGAGAC ACATTCAGGC 5461 TCCATTTAAC CAGAATAATT CCGTTATCAA AATGGAAGCG CCAAGAAGCA TCGTTGATGG 5521 CGCTTTCCCA CCATCCATCC GTCCAACACA AACCCACCTG CTTTGAAAAG ACTGCCTCAC 5581 GCAGTGACTC ACTTACCCAC CACAGCCGCA TTCTCTACCT CTGCCTCTTG TCTAGTGAAG 5641 CTGGCTCCCG AGGCTTCGGG GCCGCTCCAC AGGGACCTGA GGACGTCTCC CCGGAGACCC 5701 TGACTCAAGC TGCTCGCCGG CACAGCCGAG CACCGGCTCC CGCCTACTCA CCGCGCGGGA 5761 GGGACGGTCC CTCCTTCAGG CCAATCTCCT TATCATTGTC CCTGCTGCTG TCACCTGCTG 5821 CTGCAGACCG GGGGTCCCGT CCAGTCTCAG CCCGTCCCGT CCTCCCCCCA GCCTATTCCC 5881 GGTCCCCTCA ACCTCAGGGA CACCGCGGCC AGCTGCACCG TTGGTTTTCC AATCAAATCC 5941 TCCTCCAGGC CACCAACCAG CGCTGGCCAG GCCAATGCCG ACAATTCTTC CTCCGCCACC 6001 TGGGGCGCCT CAGCCAATCG GGAGCAGCCA TGCTGCGCAG TGCAGAACGC CATAGCGACG 6061 GATGACGCCT GCCGGGGAAT CGCCGTAGCA ACCAGAGACG CGCTAGATCC ACCACCAAGC 6121 TTGCAAGCAG AAGAGGCAGC AAGTGATGCT GAAGGCTGTG CTGCAGCCAC TTCACCGGAA 6181 CCTAGAGCTA CCTGTGAACA GGTCTCCTAC ACGTTGAAGA TTTTTCTGGA TTTAGGGTGT 6241 CTAACCTTTG ATGGGAAAGG GCAGCTTCAT CTTGGAGTGT TTACTTTCAA GAATTGCTAT 6301 GGGCTAAAAA TCAATTCTCT TTAAAAGCAA AGATTTAATC TGATAAGGAA TCAATGCAGC 6361 ATGCTAAATA CCTTTCATAT GGCATGAAAA CGTCTTCAGT TACTCAAGCC ACAGGCAATG 6421 AGAATAAGGT GCCACAGCAC CGTATTTTTC ACAAAAAGAG TGACCTGGGA TTCAAGAAAC 6481 CTGTATAGTA TAACTATCGC CAGTTCGTTT TGTGGCTTTG GGCAAGTCAC TTCACCTTTT 6541 GTGAAAGTCT GGAGCTTCTT TAAAATTTCT GAACTGCATT AGATAACGCT TTAAAAATAT 6601 ACCTGACATT CTGTAACTTT AAATAAATGA AAAATGCCAA TCTATTAAGT GCTTAATTTT 6661 GTGCCGGGCA CGATTTCAAA TTCTTTCCTT ATTTCACTTA GTTATAAAAT GGAGGCACAA 6721 GAAGTTTCCT AAGTAGTCCA GTATGGCAGA GCTAGTGATG GAGTCAGGGT AAAACCTACT 6781 TCGAGTCTGG TCTTAACCAC GATGATGCTC CTTCGACGAT TTCTTCGAGT ACTGGGCCCT 6841 GTTCAACCTC ATCTTGGTAT CACTAAACGC TCGTGTGTGT GTCTGGTGGT TACATCTTCC 6901 CAAACTGGAT TTTAAATGAA TACAGTGAAC CCTAACGTTT TTCTGGTGTG GATAGTAAGT 6961 AGGCCCATTG GATTAACAAT ACTTAAGCTT GGGTAATTGA AAGCATTTTT TAAAATGAAG 7021 ACGGTCCTAT TTTGGAAATG CAGAAAGGCG AGATGAAAGC CAAACTCTGG TGAGTATAGC 7081 AGAACTAATT AAACTTCAGA GATCTAAAAC AATCAGGATT AATGTTACCA ATAAAACAAA 7141 ACAAGGTGAC GGGGCAGGAA GACCGATGCT GAGTAGAAAA AGTATTCTGT ACATACATTC 7201 TAAATGTTAA GCAGTCGGCC TGGCTTGGTG GCTCACACCT GTAATCCTAG CACTTTGGGA 7261 GGCCGAGGCA GGAGGACCAC TTGAGGTTAG GAGTTCGAGA CCAGCCTGGC CATCATGGCC 7321 AAACCCTGTT TCTACTAAAA ACAGAAAAAT CAGCCGGGCA TGGTGGCACA TGCTAGTCCC 7381 TACTACTCAG GAGGCTGAGG CACAAGAATT ACTTGAACCC AGGAAGCAGA GGTGCAGTGA 7441 GCCAAGATCA TGCCACTGCA CTCCAGCCTG AGAGACACAG CAAGACTCTA TCTCAAAAAA 7501 TAAACATTAA GCAGTGAGAC ATGACTGCAC TGAACTTCTC ATCTTCACCA GAATACCAAT 7561 TGTGCTTCCC ATCTTTCCAC TACTCAGTAA AATTAAAGTC TTAAACTAAT GTTTTGGCCA 7621 TCCCAGAATA TTCCTGAACT TCCCTGAAAG CTGCAGCGAT TAAGAGGCAA GAATGACACT 7681 GCCTCTAAAA GACACATACA CTATCATCTC AAATGTTAAG GGTCCCTCTG TAAAATGACA 7741 CAAAATAAAA TCCCACAAGA GATCTTGCAC CAAGCTTATA GCTTATATAG CAAGGCTCAA 7801 AACTATGAAA ACATATTTTT CTAAGGCCTT TTTTTAAATG AAATGCATAA CGTAAATTAT 7861 ACAGTTCAAA AGTAAAACAG CATAATGTCC TAACTACAGC ATAAAGGTAA ggatccCACC 7921 ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 7981 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC 8041 GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC 8101 GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG 8161 TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG 8221 GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 8281 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA 8341 AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC 8401 TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC 8461 TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC 8521 AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 8581 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG 8641 GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT 8701 CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT 8761 AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC 8821 ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 8881 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC 8941 GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC 9001 GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG 9061 ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC 9121 TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 9181 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC 9241 CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA 9301 CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG 9361 CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC 9421 TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 9481 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT 9541 AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATTCTAGT TGTTTAAACG AGCTCGCTAG 9601 CCTCGAGTCT AGAGTCGACC TGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.