pT7-MBP-TEV-ERCC4-mCherry-6×Hi

pT7-MBP-TEV-ERCC4-mCherry-6×His plasmid

PVT111907

pT7-MBP-TEV-ERCC4-mCherry-6×His plasmid Information

Plasmid name: pT7-MBP-TEV-ERCC4(human)-mCherry-6×His
Alias: NM_005236.3;ERCC11; FANCQ; RAD1; XFEPS; XPF
Gene lenght: 2748
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Red
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MBP-F
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-MBP-TEV-ERCC4-mCherry-6×His plasmid Description

pT7-MBP-TEV-ERCC4-mCherry-6×His Plasmid suitable for gene expression in Escherichia coli.

pT7-MBP-TEV-ERCC4-mCherry-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atatgaaaat cgaagaaggt aaactggtaa 5101 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga 5161 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac 5221 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg 5281 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc 5341 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg 5401 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct 5461 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt 5521 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt 5581 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga 5641 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg 5701 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc 5761 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga 5821 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg 5881 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag 5941 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg 6001 aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag aaaggtgaaa 6061 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca 6121 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actgagctcg 6181 aaaatctcta cttccagagt atggagtcag ggcagccggc tcgacggatt gccatggcgc 6241 cgctgctgga gtacgagcga cagctggtgc tggaactgct cgacactgac gggctagtag 6301 tgtgcgcccg cgggctcggc gcggaccggc tcctctacca ctttctccag ctgcactgcc 6361 acccagcctg cctggtgctg gtgctcaaca cgcagccggc cgaggaggag tattttatca 6421 atcagctgaa gatagaagga gttgaacacc tccctcgccg tgtaacaaat gaaatcacaa 6481 gcaacagtcg ctatgaagtt tacacacaag gtggtgttat atttgcgaca agtaggatac 6541 ttgtggttga cttcttgact gatagaatac cttcagattt aattactggc atcttggtgt 6601 atagagccca cagaataatc gagtcttgtc aagaagcatt catcttgcgc ctctttcgcc 6661 agaaaaacaa acgtggtttt attaaagctt tcacagacaa tgctgttgcc tttgatactg 6721 gtttttgtca tgtggaaaga gtgatgagaa atctttttgt gaggaaactg tatctgtggc 6781 caaggttcca tgtagcagta aactcatttt tagaacagca caaacctgaa gttgtagaaa 6841 tccatgtttc tatgacacct accatgcttg ctatacagac tgctatactg gacattttaa 6901 atgcatgtct aaaggaacta aaatgccata acccatcgct tgaagtggaa gatttatctt 6961 tagaaaatgc tattggaaaa ccttttgaca agacaatccg ccattatctg gatcctttgt 7021 ggcaccagct tggagccaag actaaatcct tagttcagga tttgaagata ttacgaactt 7081 tgctgcagta tctctctcag tatgattgtg tcacatttct taatcttctg gaatctctga 7141 gagcaacgga aaaagctttt ggtcagaatt caggttggct gtttcttgac tccagcacct 7201 cgatgtttat aaatgctcga gcaagggttt atcatcttcc agatgccaaa atgagtaaaa 7261 aagaaaaaat atctgaaaaa atggaaatta aagaagggga agaaacaaaa aaggaactgg 7321 tcctagaaag caacccaaag tgggaggcac tgactgaagt attaaaagaa attgaggcag 7381 aaaataagga gagtgaagct cttggtggtc caggtcaagt actgatttgt gcaagtgatg 7441 accgaacatg ttcccagctg agagactata tcactcttgg agcggaggcc ttcttattga 7501 ggctctacag gaaaaccttt gagaaggata gcaaagctga agaagtctgg atgaaattta 7561 ggaaggaaga cagttcaaag agaattagga aatctcacaa aagacctaaa gacccccaaa 7621 acaaagaacg ggcttctacc aaagaaagaa ccctcaaaaa gaaaaaacgg aagttgacct 7681 taactcaaat ggtaggaaaa cctgaagaac tggaagagga aggagatgtc gaggaaggat 7741 atcgtcgaga aataagcagt agcccagaaa gctgcccgga agaaattaag catgaagaat 7801 ttgatgtaaa tttgtcatcg gatgctgctt tcggaatcct gaaagaaccc ctcactatca 7861 tccatccgct tctgggttgc agcgacccct atgctctgac aagggtacta catgaagtgg 7921 agccaagata cgtggttctt tatgacgcag agctaacctt tgttcggcag cttgaaattt 7981 acagggcgag taggcctggg aaacctctga gggtttactt tcttatatac ggaggttcaa 8041 ctgaggaaca acgctatctc actgctttgc ggaaagaaaa ggaagctttt gaaaaactca 8101 taagggaaaa agcaagcatg gttgtccctg aagaaagaga aggcagagat gaaacaaact 8161 tagacctagt aagaggcaca gcatctgcag atgtttccac tgacactcgg aaagccggtg 8221 gccaggaaca gaatggtaca cagcaaagca tagttgtgga tatgcgtgaa tttcgaagtg 8281 agcttccatc tctgatccat cgtcggggca ttgacattga acccgtgact ttagaggttg 8341 gagattacat cctcactcca gaaatgtgcg tggagcgcaa gagtatcagt gatttaatcg 8401 gctctttaaa taacggccgc ctctacagcc agtgcatctc catgtcccgc tactacaagc 8461 gtcccgtgct tctgattgag tttgacccta gcaagccttt ctctctcact tcccgaggtg 8521 ccttgtttca ggagatctcc agcaatgaca ttagttccaa actcactctt cttacacttc 8581 acttccccag actacggatt ctctggtgcc cctctcctca tgcaacggcg gagttgtttg 8641 aggagctgaa acaaagcaag ccacagcctg atgcggcgac agcactggcc attacagcag 8701 attctgaaac ccttcccgag tcagagaagt ataatcctgg tccccaagac ttcttgttaa 8761 aaatgccagg ggtgaatgcc aaaaactgcc gctccttgat gcaccacgtt aagaacatcg 8821 cagaattagc agccctgtca caagacgagc tcacgagtat tctggggaat gctgcaaatg 8881 ccaaacagct ttatgatttc attcacacct cttttgcaga agtcgtatca aaaggaaaag 8941 ggaaaaaggg aggcggtgga tcaggtggag gcggatcagg aggcggtgga tctatggtga 9001 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 9061 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 9121 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 9181 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 9241 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 9301 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 9361 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 9421 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 9481 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 9541 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 9601 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 9661 agggccgcca ctccaccggc ggcatggacg agctgtacaa gctcgagcac caccaccacc 9721 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg 9781 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc 9841 tgaaaggagg aactatatcc ggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.