pLV3-CMV-FBXO10-3×HA-Puro plas

pLV3-CMV-FBXO10-3×HA-Puro plasmid

PVT111880

pLV3-CMV-FBXO10-3×HA-Puro plasmid Information

Plasmid name: pLV3-CMV-FBXO10(human)-3×HA-Puro
Alias: NM_012166.3;FBX10; PRMT11
Gene lenght: 2868
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FBXO10-3×HA-Puro plasmid Description

pLV3-CMV-FBXO10-3×HA-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FBXO10-3×HA-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catggaggct ggtggcctcc ccttggagct gtggcgcatg 2341 atcttagcct acttgcacct tcccgacctg ggccgctgca gcctggtatg cagggcctgg 2401 tatgaactga tcctcagtct cgacagcacc cgctggcggc agctgtgtct gggttgcacc 2461 gagtgccgcc atcccaattg gcccaaccag ccagatgtgg agcctgagtc ttggagagaa 2521 gccttcaagc agcattacct tgcatccaag acatggacca agaatgcctt ggacttggag 2581 tcttccatct gcttttctct attccgccgg aggagggaac gacgtaccct gagtgttggg 2641 ccaggccgtg agtttgacag cctgggcagt gccttggcca tggccagcct gtatgaccga 2701 attgtgctct tcccaggtgt gtacgaagag caaggtgaaa tcatcttgaa ggtgcctgtg 2761 gagattgtag ggcaggggaa gttgggtgaa gtggccctgc tggccagcat tgatcagcac 2821 tgctcaacca cacgcctgtg caacctcgtc ttcacgccag cctggttctc acccatcatg 2881 tataagacaa catcaggtca cgtccagttt gacaactgca actttgagaa cgggcacatc 2941 caggtccatg gcccgggtac ttgccaagtg aagttctgta ccttcaaaaa cacccatatc 3001 ttcctgcaca acgtgcccct gtgtgtcctg gaaaactgtg aatttgtggg cagtgaaaac 3061 aactctgtga ctgttgaggg tcacccatct gcagataaga actgggccta caagtatcta 3121 ctagggctta tcaagtcctc acccactttt ctccccacag aggactctga ctttttaatg 3181 tccctggacc tagagagccg ggaccaggcc tggagcccaa agacctgtga cattgttatc 3241 gagggcagcc agagccctac cagcccagcc tctagctccc caaagccagg ctccaaggct 3301 ggctcacagg aggcagaggt gggtagtgat ggtgaaaggg tggcccagac cccggacagc 3361 agcgatggag gcctgagtcc cagcggtgag gatgaagatg aggaccagct gatgtacaga 3421 ctatcctacc aagtgcaggg cccacgccct gtattggggg gctcatttct gggcccacct 3481 ctaccaggag catccattca gctgcccagc tgcctagtgc tgaactcact gcagcaggag 3541 ctgcagaagg ataaggaggc catggcactg gccaactccg tgcagggctg cctcatccgc 3601 aagtgcctct tccgggacgg gaagggaggc gtcttcgtct gctcccacgg cagagccaag 3661 atggaaggaa acatcttccg gaacctgact tacgcagtgc ggtgtataca taatagcaag 3721 atcatcatgc tcaggaacga catttaccgc tgccgagcgt caggcatctt tcttcgcttg 3781 gagggcggtg gcttgattgc cggcaacaac atttaccaca atgcagaggc tggtgtagac 3841 atccggaaaa agtccaaccc actcatactg tgtaaccaga tccaccatgg ccttcgctct 3901 ggcattgtcg tccttggcaa tgggaaaggc atcatccgga acaatcaaat cttttccaat 3961 aaggaggctg gcatttacat cctgtaccac ggaaaccccg ttgtgagcgg gaaccacatc 4021 ttcaagggcc gtgctgccgg catagcagtg aatgagaacg gcaaaggcct catcacagaa 4081 aatgtcatcc gtgagaatca gtggggaggt gtggacatcc gccgtggagg gatccccgtt 4141 ctcaggagta acctcatctg ctttggctat tcagatggtg tggttgtggg agacgaaggc 4201 aaaggcctca tagaaggaaa taccatctac gctaacaagg gctgtggtgt gtggatgatg 4261 tcgtccagcc tcccccatgt caccagcaac cacgtcagct acaatggcct gtatggagtg 4321 gcagtattta gccagaagga tggctccagc gagttacctc gaggccacag ggctcaagag 4381 aacttcagcg aggatgggga cgccatcctc tgggagacag agctggagaa ggaggacgac 4441 ccactgcgcc ggcccatcac catagctctt gttgagtcta acagtattaa tcacaatgga 4501 gcctcaggac tctatgtcca gagcagcgag gcactgcatg tcatcaccaa tgtgatccac 4561 gcgaatgggg acagaggcat tactgtggcc cagagcagcc aacccacccg agtggccaac 4621 aacagcatct cctgcaaccg gcaaagtggg gtcaaggttg aggcccagtg caaagtggag 4681 ctccggggca atggtatcta tgacaacaga ggccacggca ttatcaccaa gggcgacagc 4741 accatcgtca ttgaaaacga tatcattggc aaccggggca gcgggctgca gctgctgccc 4801 aggtccgaca ctaaagtaat aaagaaccgg atccactcgt tccgggccta cggcatcgcc 4861 gtgcggggcc gtgccaaggc cctggtgcag gaaaacatca tcttccaggg caaaaccagt 4921 aagaccatct ttcagcagat ctcaaacaac cgagaatgca tcatgcaaaa caacaagttc 4981 ctggtcttca agaaaaagtc tgatacgtgg cgcctggtga acccaccagc acggccccac 5041 cttgaaaatt ctctcagacg tccctcggca gcccacaatg ggcagaaggt gacagccatg 5101 gcaacgagga tcacagcccg ggtggaaggt ggttaccaca gcaaccgcag tgtcttctgc 5161 accatcctgG GATCCTACCC ATACGACGTA CCTGATTATG CAACTTATCC TTATGATGTA 5221 CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAGGCGGC CGCGAAGGAT 5281 CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA 5341 GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG 5401 GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT 5461 AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT 5521 GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA 5581 TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC 5641 CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG 5701 GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA 5761 CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC 5821 GCTAGATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG 5881 CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC 5941 CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC 6001 TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC 6061 CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA 6121 GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA 6181 AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC 6241 TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT 6301 TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA 6361 CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG 6421 CCTGAGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 6481 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 6541 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 6601 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 6661 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 6721 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 6781 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC 6841 CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 6901 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 6961 TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC 7021 CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA 7081 GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA AATAAGATCT GCTTTTTGCT 7141 TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG 7201 AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT 7261 CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC 7321 TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA 7381 ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 7441 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 7501 AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA 7561 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 7621 CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGACT 7681 TTTGCAGAGA CGGCCCAAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 7741 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 7801 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 7861 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 7921 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 7981 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 8041 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 8101 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 8161 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 8221 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 8281 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 8341 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 8401 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 8461 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 8521 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 8581 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 8641 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 8701 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 8761 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 8821 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 8881 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 8941 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 9001 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 9061 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 9121 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 9181 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 9241 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 9301 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 9361 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 9421 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 9481 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 9541 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 9601 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 9661 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 9721 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 9781 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 9841 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT 9901 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 9961 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 10021 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 10081 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 10141 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT 10201 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 10261 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 10321 CGGCCAGTGC CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.